anti ccl26  (Bioss)

Journal: Cytokine

Article Title: An initial investigation into endothelial CC chemokine expression in the human rheumatoid synovium

doi: 10.1016/j.cyto.2017.05.023

Figure Lengend Snippet: Chemokines present at VWF positive vessels in RA synovia.

Article Snippet: Briefly, sections were blocked then incubated for 1 h in the primary antibodies, the working concentrations used were: anti-human mouse monoclonal CCL2 (10 μg/ml; MAB2791), mouse monoclonal CCL3 (10 μg/ml; MAB270), goat polyclonal CCL4 (10 μg/ml; AF-271-NA), goat polyclonal CCL5 (15 μg/ml; AF-278-NA), mouse monoclonal CCL11 (15 μg/ml; MAB320), goat polyclonal CCL16 (15 μg/ml; AF802), goat polyclonal CCL17 (10 μg/ml; AF364), mouse monoclonal CCL19 (20 μg/ml; MAB361), goat polyclonal CCL20 (10 μg/ml; AF360), goat polyclonal CCL21(15 μg/ml; AF366), goat polyclonal CCL24 (15 μg/ml; AF343), goat polyclonal CCL26 (10 μg/ml; AF653), mouse monoclonal CCL27 (20 μg/ml; MAB367), (all R&D Systems, UK), goat polyclonal CCL8 (2 μg/ml; Sc-1307), goat polyclonal CCL13 (4 μg/ml; Sc-9655), mouse monoclonal CCL14 (2 μg/ml; Sc-28388), goat polyclonal CCL18 (4 μg/ml; Sc-9781), goat polyclonal CCL22 (4 μg/ml; Sc-12285), goat polyclonal CCL23 (4 μg/ml; Sc-12263), goat polyclonal CCL25 (2 μg/ml; Sc-12277) and goat polyclonal CCL28 (4 μg/ml; Sc-27339) (all SantaCruz Biotechnology Inc UK), mouse monoclonal CCL1 (2.5 μg/ml; LS-C4342) and rabbit polyclonal CCL7 (2.5 μg/ml; LS-B930) (LifeSpan Biosciences, UK), rabbit polyclonal CCL10 (4 μg/ml; Orb13568), rabbit polyclonal CCL12 (2 μg/ml; Orb132384), rabbit polyclonal CCL15 (4 μg/ml; Sc-28388) (Biorbyte, UK), rabbit anti-human von Willebrand Factor (VWF) (3 μg/ml; A0082) and mouse anti-human VWF (4 μg/ml; M0616) (Dakocytomation, UK).

Techniques:

Journal: Journal of Clinical Medicine

Article Title: Aryl Hydrocarbon Receptor Activation Downregulates IL-33 Expression in Keratinocytes via Ovo-Like 1

doi: 10.3390/jcm9030891

Figure Lengend Snippet: ( A – F ) NHEKs were treated with IL-4 (10 ng/mL) in the presence or absence of either tofacitinib (100, 300, and 500 nM) or JTE-052 (100, 500, and 1000 nM) for 24 h. ( A , D ) IL-33 mRNA expression was analyzed by qRT-PCR. ( B , E ) IL-33 protein expression was analyzed by Western blotting with an anti-IL-33 antibody. The data are representative of experiments repeated three times with similar results. ( C , F ) mRNA of CCL26 in NHEKs treated with IL-4 (10 ng/mL) in the presence or absence of either tofacitinib (100, 300, and 500 nM) or JTE-052 (100, 500, and 1000 nM) for 24 h was analyzed by qRT-PCR. ( A , C , D , F ) Data are expressed as mean ± S.E.M.; n = 3 for each group. ( A , D ) Statistically significant differences between the expression of control and treated NHEKs are presented: * p < 0.05 ( A , D ). * p < 0.05 ( C , F ).

Article Snippet: Anti-human IL-33 monoclonal mouse antibody (Abcam, Cambridge, UK) and anti-human CCL26 rabbit polyclonal antibody (MyBioSource, San Diego, CA, USA) were used for immunofluorescence staining.

Techniques: Expressing, Quantitative RT-PCR, Western Blot

Journal: Journal of Clinical Medicine

Article Title: Aryl Hydrocarbon Receptor Activation Downregulates IL-33 Expression in Keratinocytes via Ovo-Like 1

doi: 10.3390/jcm9030891

Figure Lengend Snippet: ( A – F ) NHEKs were treated with IL-4 (10 ng/mL) in the presence or absence of either tofacitinib (100, 300, and 500 nM) or JTE-052 (100, 500, and 1000 nM) for 24 h. ( A , D ) IL-33 mRNA expression was analyzed by qRT-PCR. ( B , E ) IL-33 protein expression was analyzed by Western blotting with an anti-IL-33 antibody. The data are representative of experiments repeated three times with similar results. ( C , F ) mRNA of CCL26 in NHEKs treated with IL-4 (10 ng/mL) in the presence or absence of either tofacitinib (100, 300, and 500 nM) or JTE-052 (100, 500, and 1000 nM) for 24 h was analyzed by qRT-PCR. ( A , C , D , F ) Data are expressed as mean ± S.E.M.; n = 3 for each group. ( A , D ) Statistically significant differences between the expression of control and treated NHEKs are presented: * p < 0.05 ( A , D ). * p < 0.05 ( C , F ).

Article Snippet: Samples were incubated with either primary anti-human CCL26 rabbit polyclonal antibody (1:100) (MyBioSource) or primary anti-human IL-33 mouse monoclonal antibody (1:100) (Abcam) in WesternBreeze Blocker/Diluent (Invitrogen, Carlsbad, CA, USA) overnight at 4 °C.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Control